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Objective To investigate the effect of Shuyusan on the behavior, content of tumor necrosisfactor-α(TNF-α) in cortex of cerebral and neuropeptide expression in hippocampus of rats with chronic mild unpredictable stress (CMUS) depression. Methods Fifty mice were randomly divided into 5 groups:normal group, model group, fluoxetine group, and high-, low-dose group of Shuyusan. Except normal group, the other groups were given CMUS. Tail suspend test (TST) and sucrose preference test (SPT) were used to estimate animals’ behavior. The contents of TNF-αwas determined by enzyme linked immunosorbent assay (ELISA). The immunocytochemical technique was used to evaluate hippocampus neuropeptide immunoreactive expression. Results The fluoxetine group and high dose of Shuyusan group showed significant increase in the sucrose consumption and decrease in the immobility time in TST, there was significant difference compared with CMUS model group (P<0.05). The TNF-αlevel was significantly lower in fluoxetine group and high dose of Shuyusan group, there difference was significant compared with model group (P<0.05). The neuropeptide immunoreactive neurons in hippocampus area was decreased by a marked number in fluoxetine group and high dose of Shuyusan group, the average optical density decreased significantly, compared with the model group, there were significant differences (P<0.05). Conclusion Shuyusan has anti-depression likely effect, with dose-effect relationship.
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Objective To investigate the effect of Shenlongtang decoction on learning and memory ability and expression of Bcl-2 and Bax on rats hippocampus with cerebral ischemia model. Methods The cerebral ischemia animal model was established by two-vessel occlusion. Fifty rats were randomly divided into control group, model group, pisacetam group, Shenlongtang groups (low and high dosage). After modeling, rats were administrated with corresponding drugs for 28 days, the learning and memory ability was tested with Morris water maze, the number of positive cells and the integral optical density of the immunostaining on Bcl-2 and Bax protein expression in the hippocampus was detected. Results With Morris water maze test, the latency time increased significantly and times of searching submerged platform decreased significantly in the model group, the difference was significant compared with control group (P<0.01). Compared with model group, the latency time of Shenlongtang high dosage group decreased significantly and times of searching submerged platform increased significantly (P<0.05). Compared with model group, the expression of positive cells number of Bax protein expression in the hippocampus area was significantly increased (P<0.05), the Bcl-2 protein expression in the hippocampus region was significantly decreased (P<0.01). Conclusion Shenlongtang could significantly improve the learning and memory ability of cerebral ischemia rats, the mechanism may be related to regulating the expression of Bcl-2 and Bax.
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OBJECTIVE@#To explore the pathogenesis of brain damage after chronic cerebral ischemia through analysis of the differences in proteins expression in hippocampus between chronic cerebral ischemia rats and normal rats.@*METHODS@#The chronic cerebral ischemia model was established by ligating the bilateral common carotid arteries.Twenty rats were randomly divided into a model group (n=10)and a sham operation group(n=10). Four weeks later, the differences of proteins expression in hippocampus between model group and sham operation group were analyzed by two dimensional polyacryalmide gel electrophoresis and ultraflex TOF/TOF mass spectrograph.@*RESULTS@#Compared to the sham operation group, the expressions of 4 proteins were up-regulated and that of 2 proteins were down-regulated in the model group. Six proteins were identified by ultraflex TOF/TOF, which were ubiquitin carboxy-terminal hydrolase L1; Dynamin-1; TMF regulated nuclear protein-like, partial; ATP synthase; rCG50513, isoform CRA_a; and expressed sequence AU016693, isoform CRA_b.@*CONCLUSION@#Well-resolved and reproducible 2-DE patterns of chronic cerebral ischemia rats were established. Six proteins that correlate with nerve damage after chronic cerebral ischemia are identified.
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Animals , Female , Male , Rats , Brain Ischemia , Metabolism , Chronic Disease , Dynamin I , Electrophoresis, Gel, Two-Dimensional , Hippocampus , Metabolism , Proteome , Proteomics , Methods , Rats, Wistar , Ubiquitin ThiolesteraseABSTRACT
In this paper, the authors make an analysis of the historical literature during the Red Army period of the agrarian revolution war, dealing with the situation of traditional Chinese medicine in the Red Army. During that time the Red Army had created revolutionary medical hospitals, gathering herbal medicine, growing herbal plants and producing Chinese medicines. At the same time the Red Army paid great attention to enriching Chinese medicine, cultivating practitioners and treating and preventing diseases using traditional Chinese medicine. During the Red Army period there was an extreme lack of medical facilities; traditional Chinese medicine played an important role in ensuring the fighting capabilities of the Red Army units. Looking back at the Red Army period, the development of our tradition can be seen, which enables future development of traditional Chinese medicine, as well as integrated medicine.
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@#ObjectiveTo explore the effects of Shenlongjiannao capsule on the learning and memory of the cerebral ischaemia model rat as well as reactive nitrogen intermediates in the cerebral cortex.MethodsThe cerebral ischaemia animal model was established by two-vessel occlusion. 50 rats were randomly divided into 5 groups: control group with sham operation, model group, positive control group with pisacetam, Shenlongjiannao capsule groups (low and high dosage). After four weeks, the learning and memory function was tested with Morris water maze, and the contents of nitric oxide(NO) and inducible nitric-oxide synthsae(iNOS) in cerebral cortex were measured.ResultsAfter treatment, the learning and memory ability improved significently and the contents of NO and iNOS in cerebral cortex decreased in Shenlongjiannao capsule groups.ConclusionShenlonjiannao capsule can improve the learning and memory of rats with cerebral ischaemia by lowering the activity of NO and iNOS in the cerebral cortex.
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@#Objective To investigate the influence of Shenlongjiannaotang on the function of learning and memory of D-glalactose induced aging mimetic rats,and the concentrations of norepinephrine(NE),dopamine(DA) and 5-hydroxy tryptamine(5-HT) in cerebrum.Methods 50 rats were randomly divided into 5 groups: normal control group,model group,and high/middle/low dosage prescription group of Shenlongjiannaotang.The memory function was tested with Morris water maze.The concentrations of NE,DA and 5-HT were detected with HPLC with fluorescence spetrometer.Results In the model group,the latency of model mice increased,and the correct number decreased significantly compared with normal group(P<0.05).After 6 weeks administering Shenlongjiannaotang,the latency decreased and the correct number increased significantly in high dosage group,compared with those of the model group(P<0.05).The contents of NE and DA reduced in model group,and Shenlongjiannaotang could increase NE and DA contents of rats cerebrum(P<0.05).Conclusion Shenlongjiannaotang could improve the learning and memory function of D-galactose induced aging rats,which may related with the increase of NE and DA contents in cerebral tissue.The effects depend on the dosage of Shenlongjiannaotang.
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@#Objective To investigate the effect of Shenlongtang on the function of learning and memory of aging rats induced with D-glalactose. Methods 40 Wistar rats were dividied into control group, model group, treating group 1 (large dosage), treating group 2 (medial dosage), treating group 3 (lower dosage). The aging models were induced with D-galactose. The memory function was tested with passageway water maze. The activity of superoxide dismutase (SOD) and content of malodialdehyde (MDA) was measured. Results In the D-galactose model rats, the swimming time was prolonged significantly, and the correct number was decreased significantly compared with normal group (P<0.05). After Six weeks treating with Shenlongtang, the swimming time significantly shortened and the correct number increased significantly in large dosage group compared with the model group (P<0.05). The activity of SOD increased and the content of MDA decreased in large dosage group compared with that in the model group (P<0.05).Conclusion Shenlongtang can improve the learning and memory function of aging rats induced with D-glalactose and resist lipid peroxide in brain tissue.
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Objective To investigate the effect of Shuyusan on the behavior of chronic stress induced depression model rats, as well as concentrations changes of norepinephrine (NE), dopamine (DA) and serotonin (5-HT) in cerebral cortex. Method Fifty mice were randomly divided into 5 groups:normal control group, model group, amitriptyline group, and high/low dose prescription group of Shuyusan. Except normal group, the other groups were given the chronic mild unpredicted stress for 21 days. After 21 days different stimulation, open-Field test was used to estimate the behavior of rats, the concentrations of NE, DA and 5-HT were detected with fluorescence spectrometer method. Results Horizontal and vertical motion of the model group was significantly lower than that of the control group (P
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Objective To observe the protective effect of Yixintang on injury of myocardial ischemia-reperfusion with isolated working heart. Method 40 mice are randomly divided into 5 groups:control group, Yixintang group 1, Yixintang group 2, Yixintang group 3 and Verapamil group. The change of left ventricular systolic output pressure (LVSP), rate of rise left ventricular pressure (?dp/dtmax), left ventricular end diastolic pressure (LVEDP), heart rate, as well as the contain of malondialdehyde (MDA) release and activity of superoxide dismutase (SOD) were measured as investigate indicator. Results After ischemia and reperfusion in Yixintang group, the heart can be good recovery its function. The values of myocardial dynamics and hemodynamics indexes LVSP and ?dp/dtmax, in Yixintang group 3 and Verapamil group the indicator were obviously higher than those in the control group, after ischemial and reperfusion. The content of MDA in myocardial tissue in Yixintang group 3 and Verapamil group after ischemia and reperfusion was obviously lower than in other groups. It also could depress the activity of SOD, there was significant difference among Yixintang group 3, Verapamil group and control groups (P
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OBJECTIVE To investigate the effect of the Qingfei decoction in the acute upper respiratory tract infections.METHODS From Jan 2004 to Sep 2006,there were 68 cases with acute upper respiratory tract infections treated with chinese medicines.The patients were divided into 2 groups:36 cases were treated with Qingfei decoction,32 cases were treated with Shuanghuanglian granules as a control.Both of the groups were treated for 5 days,the clinical symptoms such as: fever,cough and sore throat were used to evaluate the efficacy of both groups.RESULTS Both of the groups were effective;the effective rate was 88.9% and 75.0% in the Qingfei group and in the control group,respectively.There was significant difference between 2 groups(P
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OBJECTIVE To investigate the mechanism and treatment of diabetes among elderly patients from the view of traditional Chinese medicine(TCM).METHODS On the basis of clinical experience and the achievement of the modern medicine,we expounded the mechanism of diabetes among elderly patients and treat from eight parts.RESULTS Using eight different TCM methods to treat diabetes elderly patients,we found their good curative effect with less adverse reaction,good prognosis,and very suit for old man.CONCLUSIONS TCM has large potentiality and advantage among diabetes elderly patients complicated with respiratory tract infection.
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BACKGROUND: Dyslipidemia is the important risk of cardiac cerebral vascular diseases, such as arthrosclerosis and coronary heart disease, etc.OBJECTIVE: To observe the clinical therapeutic effects of tiaozhi zengshou tang (herbal decoction) on intervention of dyslipidemia.DESIGN: Randomized, group controlled observation.SETTING: Department of Oncology, Department of Chinese Medicine and Department of Cardiology of General Hospital of Chinese PLA.PARTICIPANTS: Totally 120 cases from inpatients with dyslipidemia in Department of Chinese Medicine and Department of Cardiology of General Hospital of Chinese PLA and outpatients in Clinic of Blood lipid from February 2002 to January 2004.METHODS: Totally 120 cases of dyslipidemia were randomized into 3 groups. In Chinese herb group (43 cases), tiaozhi zengshou tang (herbal decoction) was prescribed, one dose/d, taking separately before breakfast and dinner. In western drug group (40 cases), pravastatin sodium was prescribed, 10 mg/tablet, 1 tablet/d, taking orally before sleep at night. Integrative group (37 cases) both pravastatin sodium and herbal decoction were prescribed, the dosage, composition and administration were same as previous. The treatment lasted 8 weeks, and then, the therapeutic effects on changes of blood lipid and harmful effects were observed.MAIN OUTCOME MEASURES: Comparisons of therapeutic effects and harmful effects among groups.RESULTS: 120 cases were employed in the experiment, but 27 of those were dropped out due to loss of contact and absent re-visiting in time. Total parison of therapeutic effects among groups: The effective rates in Chinese herb group and western drug group were similar (81%, 80%, P ＞ 0.05),changes of blood lipid series before and after treatment in each group: In Chinese herb group, triglyceride (TG) (2.59t1.64) mmol/L was reduced to (1.56±0.72) mmol/L, serum total cholesterol (TC) (5.30±1.71) mmol/L was reduced to (4.35±0.85) mmol/L (P ＜ 0.01) and HDL-Cholesterol (HDL-Ch)(1.32±0.37) mmol/L was increased to (1.50±0.22) mmol/L (P ＜ 0.05). In western drug group, TG (2.84±1.50) mmol/L was reduced to (2.04±0.98) mmol/L,serum TC (5.50±1.22) mmol/L was reduced to (4.71±0.89) mmol/L (P ＜ 0.05,P ＜ 0.01) and APoA1 (1.24±0.21) g/L was increased to (1.49±0.15) g/L (P ＜ 0.01). In integrative group, the therapeutic effects were significant in re ducing TG, TC, LDL-Ch and APoB and increasing APoA1 and HDL-Ch harmful effects in Chinese herb group were less than western drug group (1 case, 7 cases).CONCLUSION: Tiaozhi zengshou tang provides definitely clinical therapeutic effects in dyslipidemia and presents low incident of harmful effects.
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Because of the effects of misbalance of Qi/Blood and Yin/Yang and abuse of medicine,food and emotion,phlegm and static blood syndrome is easy to be made in the course of pathological changes of migraine.Phlegm and static blood syndrome is the important factor in the occurrence and development of migraine. In the whole period of migraine it is showing not only stabbing pain immovable for a long time,but also showing languor and scarce of appetite;The method of dissipating blood stasis and phlegm should be used effectively.
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Objective: In order to have more direction to treat senile diseases,the feature of diseases and the principle of medication are investigated.Methods: Through combining the experiences of prevention and cure of senile diseases,we can conclude and analyze from its characteristics of imbalance of YIN and YANG,clumsy of reaction,deterioration of entrails,inclusion of excess and deficiency et al.Results: Aiming its physiological and pathological feature,we propose the standpoint that we should limit reinforcing and reducing,protect gastric-qi and prescribe containing minerals.Conclusion: It has evident specificity when we diagnose and prescribe for the aged,and can not equate that to the young.
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OBJECTIVE Epidemic diseases have been recorded in traditional chinese medicine(TCM) for a long time,this paper describes the cognition of TCM on treatment and prevention for epidemic diseases.METHODS This topic was discussed from etiology,pathogenesis,and the process of epidemic diseases in TCM.TCM holds that the occurrence of epidemic diseases is the result of the combined effects of the seasonal and environmental factors,and the internal factor of human body and the epidemic pathogenic factors play the same important role in the process of epidemic diseases.The diagnosis patterns of syndrome differentiation were six meridians,differentiation of Wei,Qi,Ying,and blood,and syndrome differentiation of Sanjiao.RESULTS TCM emphasizes the harmful effects of pathogenic factors on human body,while it pays more attention to the sufficiency of vital Qi defeating the pathogenic factors and the maintenance of human body positive vital Qi.Beside the treatment method,also have some preventive methods been described in TCM,such as different sterilization methods.CONCLUSIONS TCM has its unique system in struggle against epidemic diseases.It is useful and effective in preventing and treating the epidemic diseases.
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Objective:To explore the influence of Xiaokehuayu Pills on blood glucose,superoxide dismutase(SOD) activity and malonaldehyde(MDA) of diabetic complicating hyperlipemia rats.Methods:Diabetes rat model was established by injection of alloxan with high fat diet.After the model formed,60 rats were been divided into 6 groups:normal control group,model group,high/middle/low dose groups of Xiaokehuayu Pills and Yuquan Pills group.The blood glucose and SOD activity and MDA content was measured before and after 6 weeks treatment.Results:The blood glucose in model group was signif icantly higher than that in normal control group(P
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Objective To investigate the effect of Yangyinjiandu decoction for acute radiation esophagitis.Methods Lung cancer and mediastinal tumor treated by radiotherapy(portals including oesophagus to a radical tumoricidal dose≥40Gy, with≥10cm oesophagus include in radiotherapy).Patients were randomly divided according to the time of acceptance into the treatment group and the control group.All parameters of the two groups were basically similar and comparable.The treatment group was given Yangyinjiandu decoction(one dose daily,water decoction,200ml,twice a day) taken in the morning and in the evening before the end of radiotherapy.The control group was given oral vitamin C tablet 100mg once a day before the end of radiotherapy.Five days after radiotherapy,the control group took oral prednisone 5mg three times a day and amoxicillin 5g three times a day.All the above medicines were continued for 7 days.Results The commencement of complication was 14.86?0.34 days in the treatment group and 13.55?0.26 days in the control group(P
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Objective To investigate the clinical effects of Shenlong decoction(main ingredients consisted of ginsent root,radix polygonimultiflori,Pheretima,Ligusticum wallichii,Fructas Alpiniae oryphyllee etc.)on the treatment of cerebral vascular dementia (VD)in the elderly patients.Methods VD in all the patients(age:72.1?5.3 years old)was diagnosed according to the APA criteria, and the cerebrovascular diseases were confirmed by CT or MRI.68 patients were divided into 2 groups randomly.The experimental group consisting of 36 patients was given Chinese traditional medicine,Shenlong decoction,orally 500ml q.i.d.for 3 months,and the other 32 patients were given acetamide pyrrolidone(piracetam)orally 1.6g,tid for 3 months as a control group.The MMSE(Mini Mental State Examination)and BBS(Bless Behaviour Schedule)were used to evaluate the cognitive function and the daily activity in those patients be- fore and after treatment.Results After treatment,the scores of cognition and daily activity were improved in all the patients with VD. The effective rate in the Shenlong group was 52.8%,and 53.13% in the piracetam group.There was no significant difference in the im- provement of the short time memory and orientation ability between the two groups.However,compared with the control group,the score of daily activity was significantly improved in the Shenlong decoction group only(P
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Objective To observe the changes in sublingual collateral vessels of elderly patients with diabetes. Methods Sublingual collateral vessels of 88 patients with diabetes were observed and compared with that of 76 normal people. Results The changes in sublingual collateral vessels of patients with diabetes were more serious than that of normal people (u=4.174, P
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Objective To evaluate the treatment of ulcerative colitis with combined Chinese oral herbs and herbs enema with western medicine. Methods 105 patients with ulcerative colitis diagnosed by colonoscopy were randomly divided into two groups: traditional combining with western medicine treatment group (treatment group) with 65 patients, and western medicine treatment group (control group) with 40 patients. In the treatment group the treatment consisted of oral herbs and enema herbs as well as sulfasalazine, while in the control group the patients received sulfasalazine only. After two courses of treatment, the immediate cure rate and cure rate after 2 years were assessed. Results The immediate cure rate and total efficacious rate of treatment group were 52% and 94.64% respectively, while those of control group were 36.2% and 82.5%, respectively. There was significant difference between two groups in the effective rate (P